Engineering of/with Lipases: Proceedings of the NATO Advanced Study Institute, P

Description: Engineering of/with Lipases by F.X. Malcata Proceedings of the NATO Advanced Study Institute, Povoa de Varzim, Portugal, May 22-June 2, 1995 FORMAT Hardcover LANGUAGE English CONDITION Brand New Publisher Description The innovative uses of lipases in a wide variety of organic syntheses and the modification of existing fats and oils have increased exponentially over the last five years, due to the increasing availability of lipases from (genetically engineered) microbial sources, coupled with their special capacity to act as catalysts at hydrophilic/hydrophobic interfaces. As a result of the structural characterisation performed during the same period, applications of these lipases can now be developed in a much more rational way. Engineering of/with Lipases presents two major topics: the design and production of lipases with desired, preselected properties, and the use of lipases for desired applications. Audience: Doctoral and post-doctoral crystallographers, biochemists, geneticists and enzyme kineticists. Food, chemical and biochemical engineers. The former will learn about the practical aims and constraints associated with industrial applications of lipases, enabling them to design lipases for specific purposes.The latter will learn how to take advantage of the structural knowledge of lipases and their metabolic genesis to better design media, processes and products in terms of biochemical and technical feasibility. Table of Contents Overview.- Engineering of/with lipases: scope and strategies.- Selectivities: fundamentals and applications.- Selectivity of lipases: types and determination.- Selectivity of lipases: developmental physiology aspects — the role of lipase selectivity in the digestion of milk fat.- Lipase-catalyzed reactions for the fractionation of fatty acids.- Lipase specificities towards fatty acids: erucic acid and ?-linolenic acid.- Synthesis of chiral drugs using lipases.- Structure.- Structural characterization of a prokaryotic lipase.- Candida rugosa lipase isozymes: cloning, sequencing, analysis of the substrate binding pocket.- Structure/function relationships.- Lipases and esterases: some evolutionary and protein engineering aspects.- The kinetics, specificities and structural features of lipases.- Action of lipases.- Molecular engineering.- Strategies and design of mutations in lipases.- Protein engineering of microbial lipases with industrial interest.- Protein engineering of a fungal lipase: towards expression of a recombinant Candida rugosa lipase.- Interfacial activation-based molecular bioimprinting: towards a more rational use of lipolytic enzymes in nonaqueous media.- Modulation of activity/stability properties of lipase from Pseudomonas fluorescens by multipoint covalent immobilization on glyoxyl-supports.- Utilization of unfolding/refolding strategies for reactivation of immobilized derivatives of lipases after inactivation by organic solvents.- Kinetics and thermodynamics.- Chromatographic and spectrometric methods for the determination of the chemical composition of lipase-modified fats.- Kinetics of lipase catalyzed reactions in non conventional media.- Kinetics and stability of cutinase immobilized in reversed micelles and zeolites.- Thermodynamic principlesof enzymatic esterification.- Role of water.- Lipases in biphasic solid/liquid media.- Engineering of/with lipases: importance of water in new biocatalytic processes — the solid/gas catalysis example.- Applications.- Plant lipases in lipid biotransformations.- Lipase-catalyzed reactions under microaqueous conditions.- Wax synthesis by esterification and alcoholysis.- Glyceride synthesis from free fatty acids and glycerol.- Reactors.- Reactors with immobilized lipase: mathematical modelling.- Lipase membrane reactor for continuous hydrolysis of tallow.- Membrane bioreactor design to force equilibrium towards a favourable product yield.- Reversed micellar membrane bioreactor.- Bioreactors for continuous enzymatic esterification with insitu water activity control - I.- Bioreactors for continuous enzymatic esterification with insitu water activity control - II.- Scale-up of two-phase membrane reactors: use of free and immobilised lipase.- Separation/reaction.- Separation and purification of lipases by liquid-liquid extraction processes.- Lipases in water-in-oil microemulsions, organogels and Winsor II systems: aspects of reactivity and separation science.- Integration of reaction and separation with lipases: biocatalytic distillation.- Reaction/separation process in supercritical CO2 using lipases. Long Description This book attempts to (i) systematically review the state of the art pertaining to the knowledge on the various levels of structure and catalytic function of Iipases, and (ii) discuss in a simultaneously pragmatic and integrated manner the design and modification of Iipases and the technological aspects of the utilization of Iipases for desired practical purposes. In preliminary assessments of whether the current knowledge of Jipases (and users thereof) would deserve (and benefit from) comprehensive academic and scholastic efforts eventually leading to the production of a formal book, four major issues were addressed: -Need. Although several applications of Iipases have been developped throughout the latest two decades, innovative uses of these enzymes for a wide variety of organic syntheses and modification of existing fats and oils have increased exponentially during the latest five years. Reasons which may partly account for this trend are the increasing availability of Jipases from (genetically engineered) microbial sources coupled with their special capacity to act as catalysts at hydrophilic / hydrophobic interfaces. As a result of the structural characterisation effected during the same period, applications of Jipases can now be developed in a much more rational fashion; however, this purpose will not be fully achieved unless careful attempts to comprehensively clarify and integrate such advances are developed. -Importance. Once viewed as solutions in search of problems, Iipases have conquered a special position in the field of biotechnology since the advent of enzyme Details ISBN0792340035 Language English ISBN-10 0792340035 ISBN-13 9780792340034 Media Book Format Hardcover Series Number 317 Year 1996 Publication Date 1996-03-31 Place of Publication Dordrecht Publisher Springer Short Title ENGINEERING OF/WITH LIPASES 19 Pages 618 Subtitle Proceedings of the NATO Advanced Study Institute, Povoa De Varzim, Portugal, May 22-June 2, 1995 Imprint Springer Country of Publication Netherlands DOI 10.1023/b127431;10.1007/978-94-009-1671-5 Edited by F.X. Malcata Author F.X. Malcata Edition Description 1996 ed. Series NATO Science Series E: Edition 1996th Alternative 9789401072489 DEWEY 574.19253 Illustrations XVI, 618 p. Audience Postgraduate, Research & Scholarly We've got this At The Nile, if you're looking for it, we've got it. 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